pgex-6p-1 vector (Cytiva Europe)
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Cytiva Europe
pgex-6p-1 vector
Pgex 6p 1 Vector, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 95/100, based on 1244 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgex+6p/PGEX-6P-1+Vector/custom%4028954648%4042429626
Average 95 stars, based on 1244 article reviews
Pgex 6p 1 Vector, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 95/100, based on 1244 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgex+6p/PGEX-6P-1+Vector/custom%4028954648%4042429626
Average 95 stars, based on 1244 article reviews
pgex-6p-1 vector - by Bioz Stars,
2026-09
95/100 stars
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High Molecular Weight:Article Title: Ferritin Blocks Inhibitory Effects of Two-Chain High Molecular Weight Kininogen (HKa) on Adhesion and Survival Signaling in Endothelial Cells Article Snippet: Endotoxin was removed using Detoxi-gel endotoxin removing gel (Thermo Scientific) and quantified using QCL-1000 Quantitative Chromogenic LAL Assay (Lonza). .. cDNA encoding domain 5 of the human high molecular weight kininogen protein (HK5) was subcloned into the Expressing:Article Title: Ferritin Blocks Inhibitory Effects of Two-Chain High Molecular Weight Kininogen (HKa) on Adhesion and Survival Signaling in Endothelial Cells Article Snippet: Endotoxin was removed using Detoxi-gel endotoxin removing gel (Thermo Scientific) and quantified using QCL-1000 Quantitative Chromogenic LAL Assay (Lonza). .. cDNA encoding domain 5 of the human high molecular weight kininogen protein (HK5) was subcloned into the Article Title: The Protein Kinase aPKC as Well as the Small GTPases RhoA and Cdc42 Regulates Neutrophil Chemotaxis Partly by Recruiting the ROCK Kinase to the Leading Edge. Article Snippet: .. The cDNAs were ligated to the following expression vectors: pEF- BOS (Mizushima and Nagata 1990) or pcDNA3.1(+) (Thermo Fisher Scientific) for expression in mammalian cells; and Article Title: The Identification of a Small Molecule Compound That Reduces HIV-1 Nef-Mediated Viral Infectivity Enhancement Article Snippet: .. The cDNA containing the motif was amplified by PCR using the following primers ( 5′-GGATCCGTGGGTTTTCCAGT-3′ and 5′-GTCGACCTATAAAGCTGCCT-3′ ), cloned into the pCR2.1 vector (Invitrogen), sequenced using the BigDye Terminator v3.1 Cycle Sequencing kit (Applied Biosystems) and the ABI PRISM 3100 Genetic Analyzer (Applied Biosystems), and cloned into the Article Title: Kaposi’s Sarcoma-Associated Herpesvirus ORF21 Enhances the Phosphorylation of MEK and the Infectivity of Progeny Virus Article Snippet: .. Amplified ORF21 DNA was cloned into the Article Title: TMEM25 is a Par3-binding protein that attenuates claudin assembly during tight junction development Article Snippet: The cDNAs encoding human TMEM25, claudin-1, claudin-2, claudin-4, claudin-15, claudin-25, occludin, and JAM-A were prepared by PCR using Human Multiple Tissue cDNA panels (BD Biosciences); the cDNA for human Par3 was cloned as described previously (Kohjima et al, ). .. The cDNA fragments for various regions of Par3 and TMEM25 (summarized in Fig. ) were amplified by PCR using specific primers and ligated to the indicated expression vector: Par3-PDZ1 (amino acid residues 221–321), Par3-PDZ2 (residues 408–510), Par3-PDZ3 (residues 525–626), Par3-PDZ2/3 (residues 408–626), TMEM25-262–366 (residues 262–366), and TMEM25-262–362 (residues 262–362) to pGADGH or pGBK, a modified pGBT vector, for yeast two-hybrid experiments; TMEM25-ECR (residues 27–220), TMEM25-262–366 (residues 262–366), TMEM25-262–362 (residues 262–362), TMEM25-355–366 (residues 355–366), and TMEM25-355–362 (residues 355–362) to Plasmid Preparation:Article Title: Ferritin Blocks Inhibitory Effects of Two-Chain High Molecular Weight Kininogen (HKa) on Adhesion and Survival Signaling in Endothelial Cells Article Snippet: Endotoxin was removed using Detoxi-gel endotoxin removing gel (Thermo Scientific) and quantified using QCL-1000 Quantitative Chromogenic LAL Assay (Lonza). .. cDNA encoding domain 5 of the human high molecular weight kininogen protein (HK5) was subcloned into the Article Title: The Identification of a Small Molecule Compound That Reduces HIV-1 Nef-Mediated Viral Infectivity Enhancement Article Snippet: .. The cDNA containing the motif was amplified by PCR using the following primers ( 5′-GGATCCGTGGGTTTTCCAGT-3′ and 5′-GTCGACCTATAAAGCTGCCT-3′ ), cloned into the pCR2.1 vector (Invitrogen), sequenced using the BigDye Terminator v3.1 Cycle Sequencing kit (Applied Biosystems) and the ABI PRISM 3100 Genetic Analyzer (Applied Biosystems), and cloned into the Article Title: Kaposi’s Sarcoma-Associated Herpesvirus ORF21 Enhances the Phosphorylation of MEK and the Infectivity of Progeny Virus Article Snippet: .. Amplified ORF21 DNA was cloned into the Article Title: TMEM25 is a Par3-binding protein that attenuates claudin assembly during tight junction development Article Snippet: The cDNAs encoding human TMEM25, claudin-1, claudin-2, claudin-4, claudin-15, claudin-25, occludin, and JAM-A were prepared by PCR using Human Multiple Tissue cDNA panels (BD Biosciences); the cDNA for human Par3 was cloned as described previously (Kohjima et al, ). .. The cDNA fragments for various regions of Par3 and TMEM25 (summarized in Fig. ) were amplified by PCR using specific primers and ligated to the indicated expression vector: Par3-PDZ1 (amino acid residues 221–321), Par3-PDZ2 (residues 408–510), Par3-PDZ3 (residues 525–626), Par3-PDZ2/3 (residues 408–626), TMEM25-262–366 (residues 262–366), and TMEM25-262–362 (residues 262–362) to pGADGH or pGBK, a modified pGBT vector, for yeast two-hybrid experiments; TMEM25-ECR (residues 27–220), TMEM25-262–366 (residues 262–366), TMEM25-262–362 (residues 262–362), TMEM25-355–366 (residues 355–366), and TMEM25-355–362 (residues 355–362) to Article Title: Molecular modeling and interaction between Arabidopsis sulfite oxidase and the GW motif of Turnip crinkle virus coat protein. Article Snippet: .. 353 354 Plasmid constructs 355 Inserts corresponding to cDNA sequences of TCV CP full length (FL), TCV 356 CP R domain (R), TCV CP S domain (S), TCV CP P domain (P), and TCV CP S and 357 Jo urn al Pr eroo f 20 P domains (SP) were cloned into the EcoRI and XhoI restriction sites of Recombinant:Article Title: Ferritin Blocks Inhibitory Effects of Two-Chain High Molecular Weight Kininogen (HKa) on Adhesion and Survival Signaling in Endothelial Cells Article Snippet: Endotoxin was removed using Detoxi-gel endotoxin removing gel (Thermo Scientific) and quantified using QCL-1000 Quantitative Chromogenic LAL Assay (Lonza). .. cDNA encoding domain 5 of the human high molecular weight kininogen protein (HK5) was subcloned into the Purification:Article Title: Ferritin Blocks Inhibitory Effects of Two-Chain High Molecular Weight Kininogen (HKa) on Adhesion and Survival Signaling in Endothelial Cells Article Snippet: Endotoxin was removed using Detoxi-gel endotoxin removing gel (Thermo Scientific) and quantified using QCL-1000 Quantitative Chromogenic LAL Assay (Lonza). .. cDNA encoding domain 5 of the human high molecular weight kininogen protein (HK5) was subcloned into the Amplification:Article Title: The Identification of a Small Molecule Compound That Reduces HIV-1 Nef-Mediated Viral Infectivity Enhancement Article Snippet: .. The cDNA containing the motif was amplified by PCR using the following primers ( 5′-GGATCCGTGGGTTTTCCAGT-3′ and 5′-GTCGACCTATAAAGCTGCCT-3′ ), cloned into the pCR2.1 vector (Invitrogen), sequenced using the BigDye Terminator v3.1 Cycle Sequencing kit (Applied Biosystems) and the ABI PRISM 3100 Genetic Analyzer (Applied Biosystems), and cloned into the Article Title: Kaposi’s Sarcoma-Associated Herpesvirus ORF21 Enhances the Phosphorylation of MEK and the Infectivity of Progeny Virus Article Snippet: .. Amplified ORF21 DNA was cloned into the Article Title: TMEM25 is a Par3-binding protein that attenuates claudin assembly during tight junction development Article Snippet: The cDNAs encoding human TMEM25, claudin-1, claudin-2, claudin-4, claudin-15, claudin-25, occludin, and JAM-A were prepared by PCR using Human Multiple Tissue cDNA panels (BD Biosciences); the cDNA for human Par3 was cloned as described previously (Kohjima et al, ). .. The cDNA fragments for various regions of Par3 and TMEM25 (summarized in Fig. ) were amplified by PCR using specific primers and ligated to the indicated expression vector: Par3-PDZ1 (amino acid residues 221–321), Par3-PDZ2 (residues 408–510), Par3-PDZ3 (residues 525–626), Par3-PDZ2/3 (residues 408–626), TMEM25-262–366 (residues 262–366), and TMEM25-262–362 (residues 262–362) to pGADGH or pGBK, a modified pGBT vector, for yeast two-hybrid experiments; TMEM25-ECR (residues 27–220), TMEM25-262–366 (residues 262–366), TMEM25-262–362 (residues 262–362), TMEM25-355–366 (residues 355–366), and TMEM25-355–362 (residues 355–362) to Polymerase Chain Reaction:Article Title: The Identification of a Small Molecule Compound That Reduces HIV-1 Nef-Mediated Viral Infectivity Enhancement Article Snippet: .. The cDNA containing the motif was amplified by PCR using the following primers ( 5′-GGATCCGTGGGTTTTCCAGT-3′ and 5′-GTCGACCTATAAAGCTGCCT-3′ ), cloned into the pCR2.1 vector (Invitrogen), sequenced using the BigDye Terminator v3.1 Cycle Sequencing kit (Applied Biosystems) and the ABI PRISM 3100 Genetic Analyzer (Applied Biosystems), and cloned into the Article Title: TMEM25 is a Par3-binding protein that attenuates claudin assembly during tight junction development Article Snippet: The cDNAs encoding human TMEM25, claudin-1, claudin-2, claudin-4, claudin-15, claudin-25, occludin, and JAM-A were prepared by PCR using Human Multiple Tissue cDNA panels (BD Biosciences); the cDNA for human Par3 was cloned as described previously (Kohjima et al, ). .. The cDNA fragments for various regions of Par3 and TMEM25 (summarized in Fig. ) were amplified by PCR using specific primers and ligated to the indicated expression vector: Par3-PDZ1 (amino acid residues 221–321), Par3-PDZ2 (residues 408–510), Par3-PDZ3 (residues 525–626), Par3-PDZ2/3 (residues 408–626), TMEM25-262–366 (residues 262–366), and TMEM25-262–362 (residues 262–362) to pGADGH or pGBK, a modified pGBT vector, for yeast two-hybrid experiments; TMEM25-ECR (residues 27–220), TMEM25-262–366 (residues 262–366), TMEM25-262–362 (residues 262–362), TMEM25-355–366 (residues 355–366), and TMEM25-355–362 (residues 355–362) to Clone Assay:Article Title: The Identification of a Small Molecule Compound That Reduces HIV-1 Nef-Mediated Viral Infectivity Enhancement Article Snippet: .. The cDNA containing the motif was amplified by PCR using the following primers ( 5′-GGATCCGTGGGTTTTCCAGT-3′ and 5′-GTCGACCTATAAAGCTGCCT-3′ ), cloned into the pCR2.1 vector (Invitrogen), sequenced using the BigDye Terminator v3.1 Cycle Sequencing kit (Applied Biosystems) and the ABI PRISM 3100 Genetic Analyzer (Applied Biosystems), and cloned into the Article Title: Kaposi’s Sarcoma-Associated Herpesvirus ORF21 Enhances the Phosphorylation of MEK and the Infectivity of Progeny Virus Article Snippet: .. Amplified ORF21 DNA was cloned into the Article Title: Molecular modeling and interaction between Arabidopsis sulfite oxidase and the GW motif of Turnip crinkle virus coat protein. Article Snippet: .. 353 354 Plasmid constructs 355 Inserts corresponding to cDNA sequences of TCV CP full length (FL), TCV 356 CP R domain (R), TCV CP S domain (S), TCV CP P domain (P), and TCV CP S and 357 Jo urn al Pr eroo f 20 P domains (SP) were cloned into the EcoRI and XhoI restriction sites of Sequencing:Article Title: The Identification of a Small Molecule Compound That Reduces HIV-1 Nef-Mediated Viral Infectivity Enhancement Article Snippet: .. The cDNA containing the motif was amplified by PCR using the following primers ( 5′-GGATCCGTGGGTTTTCCAGT-3′ and 5′-GTCGACCTATAAAGCTGCCT-3′ ), cloned into the pCR2.1 vector (Invitrogen), sequenced using the BigDye Terminator v3.1 Cycle Sequencing kit (Applied Biosystems) and the ABI PRISM 3100 Genetic Analyzer (Applied Biosystems), and cloned into the Article Title: TMEM25 is a Par3-binding protein that attenuates claudin assembly during tight junction development Article Snippet: The cDNAs encoding human TMEM25, claudin-1, claudin-2, claudin-4, claudin-15, claudin-25, occludin, and JAM-A were prepared by PCR using Human Multiple Tissue cDNA panels (BD Biosciences); the cDNA for human Par3 was cloned as described previously (Kohjima et al, ). .. The cDNA fragments for various regions of Par3 and TMEM25 (summarized in Fig. ) were amplified by PCR using specific primers and ligated to the indicated expression vector: Par3-PDZ1 (amino acid residues 221–321), Par3-PDZ2 (residues 408–510), Par3-PDZ3 (residues 525–626), Par3-PDZ2/3 (residues 408–626), TMEM25-262–366 (residues 262–366), and TMEM25-262–362 (residues 262–362) to pGADGH or pGBK, a modified pGBT vector, for yeast two-hybrid experiments; TMEM25-ECR (residues 27–220), TMEM25-262–366 (residues 262–366), TMEM25-262–362 (residues 262–362), TMEM25-355–366 (residues 355–366), and TMEM25-355–362 (residues 355–362) to Modification:Article Title: TMEM25 is a Par3-binding protein that attenuates claudin assembly during tight junction development Article Snippet: The cDNAs encoding human TMEM25, claudin-1, claudin-2, claudin-4, claudin-15, claudin-25, occludin, and JAM-A were prepared by PCR using Human Multiple Tissue cDNA panels (BD Biosciences); the cDNA for human Par3 was cloned as described previously (Kohjima et al, ). .. The cDNA fragments for various regions of Par3 and TMEM25 (summarized in Fig. ) were amplified by PCR using specific primers and ligated to the indicated expression vector: Par3-PDZ1 (amino acid residues 221–321), Par3-PDZ2 (residues 408–510), Par3-PDZ3 (residues 525–626), Par3-PDZ2/3 (residues 408–626), TMEM25-262–366 (residues 262–366), and TMEM25-262–362 (residues 262–362) to pGADGH or pGBK, a modified pGBT vector, for yeast two-hybrid experiments; TMEM25-ECR (residues 27–220), TMEM25-262–366 (residues 262–366), TMEM25-262–362 (residues 262–362), TMEM25-355–366 (residues 355–366), and TMEM25-355–362 (residues 355–362) to Mutagenesis:Article Title: TMEM25 is a Par3-binding protein that attenuates claudin assembly during tight junction development Article Snippet: The cDNAs encoding human TMEM25, claudin-1, claudin-2, claudin-4, claudin-15, claudin-25, occludin, and JAM-A were prepared by PCR using Human Multiple Tissue cDNA panels (BD Biosciences); the cDNA for human Par3 was cloned as described previously (Kohjima et al, ). .. The cDNA fragments for various regions of Par3 and TMEM25 (summarized in Fig. ) were amplified by PCR using specific primers and ligated to the indicated expression vector: Par3-PDZ1 (amino acid residues 221–321), Par3-PDZ2 (residues 408–510), Par3-PDZ3 (residues 525–626), Par3-PDZ2/3 (residues 408–626), TMEM25-262–366 (residues 262–366), and TMEM25-262–362 (residues 262–362) to pGADGH or pGBK, a modified pGBT vector, for yeast two-hybrid experiments; TMEM25-ECR (residues 27–220), TMEM25-262–366 (residues 262–366), TMEM25-262–362 (residues 262–362), TMEM25-355–366 (residues 355–366), and TMEM25-355–362 (residues 355–362) to Construct:Article Title: Molecular modeling and interaction between Arabidopsis sulfite oxidase and the GW motif of Turnip crinkle virus coat protein. Article Snippet: .. 353 354 Plasmid constructs 355 Inserts corresponding to cDNA sequences of TCV CP full length (FL), TCV 356 CP R domain (R), TCV CP S domain (S), TCV CP P domain (P), and TCV CP S and 357 Jo urn al Pr eroo f 20 P domains (SP) were cloned into the EcoRI and XhoI restriction sites of Negative Control:Article Title: DNA processing by the Kaposi's sarcoma-associated herpesvirus alkaline exonuclease SOX contributes to viral gene expression and infectious virion production. Article Snippet: Reactions were conducted under conditions of enzyme excess (250 nM) and fluorescence intensity was measured using a Spark multimode plate reader (TECAN) at 484 nM excitation and 522 nM emission. .. A reaction without enzyme served as a negative control to account for a gradual decrease in fluorescence over time, and a sample with 0.005 nM Fluorescence:Article Title: DNA processing by the Kaposi's sarcoma-associated herpesvirus alkaline exonuclease SOX contributes to viral gene expression and infectious virion production. Article Snippet: Reactions were conducted under conditions of enzyme excess (250 nM) and fluorescence intensity was measured using a Spark multimode plate reader (TECAN) at 484 nM excitation and 522 nM emission. .. A reaction without enzyme served as a negative control to account for a gradual decrease in fluorescence over time, and a sample with 0.005 nM Positive Control:Article Title: DNA processing by the Kaposi's sarcoma-associated herpesvirus alkaline exonuclease SOX contributes to viral gene expression and infectious virion production. Article Snippet: Reactions were conducted under conditions of enzyme excess (250 nM) and fluorescence intensity was measured using a Spark multimode plate reader (TECAN) at 484 nM excitation and 522 nM emission. .. A reaction without enzyme served as a negative control to account for a gradual decrease in fluorescence over time, and a sample with 0.005 nM |